On another level: the role of ubiquitination in endothelial inflammation

Abstract

Endothelial cells form the lining of the bloodvessel. They form the fysical barrier between the blood and tissue, they provide nutrient exchange and prevent coagulation. Upon inflammation, endothelial cells are activated, resulting in a variety of molecular and celullar responses. Previously, the endothelial response to IFN and TNF has been investigated and it was found that both lead to a unique response. Additionally, costimulation of both cytokines seemed to enhance this response synergistically. Here, we examine the role of ubiquitin in TNF induces endothelial inflammation. We describe our optimized protocol show upregulated ubiquitin sites that are in consensus with previous studies in other cell types.