A modelling approach to uncovering hostspecific interactions by jumbo phage FLC4-4c

Abstract

Recent efforts in developing bacteriophage-based treatments for B. glumae infection have identified a Jumbo phage FLC4-4c as a potential candidate. This Jumbo phage has a wide host range and is capable of infecting both Burkholderia plantarii and Burkholderia gladioli. However, while performing plaque assays on B. plantarii bacterial lawns, the phage suddenly loses its ability to infect. In this project, we validate the experimentally found abrupt disappearance in plaque formation. Dispite the implementation of alternative density-dependent resistance mechanisms in the bacteria, we were unable to recreate the abrupt disappearance as observed previously