Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae – a leap into disease pathophysiology

Abstract

Introduction Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available. Objective The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae. Methods Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes. Results Significant epithelial necrosis, increased levels of L-citrulline and IL-1alfa were observed on explants infected with B. hyodysenteriae Conclusions The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1alfa and NO.